NID1 | PDGFRB (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human NID1 | PDGFRB, hFc Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 21.5 ng/mL determined by ELISA.
SEC-HPLC
Human NID1 | PDGFRB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Tris-Bis PAGE
Human NID1 | PDGFRB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human NID1 | PDGFRB (P06756-1 | P05106-1 Phe31 - Val992 & Gly27 - Asp718) partial recombinant protein with His tag and Flag tag at C-terminus expressed in HEK293 cells.
Sequence
Phe31 - Val992 & Gly27 - Asp718
Host
Human
Theoretical MW (kDa)
131.9
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 21.5 ng/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
Human NID1 | PDGFRB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Tris-Bis PAGE
Human NID1 | PDGFRB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human NID1 | PDGFRB, hFc Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 21.5 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — NID1
Entrez GeneID
4811Protein Accession#
P06756-1 | P05106-1Gene Name
NID1
Gene Alias
NID
Gene Description
nidogen 1
Omim ID
131390Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq
Other Designations
OTTHUMP00000037970|enactin|entactin
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Gene Info — PDGFRB
Entrez GeneID
5159Protein Accession#
P06756-1 | P05106-1Gene Name
PDGFRB
Gene Alias
CD140B, JTK12, PDGF-R-beta, PDGFR, PDGFR1
Gene Description
platelet-derived growth factor receptor, beta polypeptide
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq
Other Designations
beta platelet-derived growth factor receptor|platelet-derived growth factor receptor beta|soluble PDGFRb variant 1
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Interactome
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Pathway
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Disease
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