TM4SF1 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human TM4SF1 VLP at 5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-TM4SF1 Antibody, hFc Tag with the EC50 of 6.3 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human TM4SF1 VLP is greater than 95% as determined by SEC-HPLC.
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Specification
Product Description
Human TM4SF1 (P30408, 1 a.a. - 202 a.a.) full recombinant protein expressed in HEK294 cells.
Sequence
MCYGKCARCIGHSLVGLALLCIAANILLYFPNGETKYASENHLSRFVWFFSGIVGGGLLMLLPAFVFIGLEQDDCCGCCGHENCGKRCAMLSSVLAALIGIAGSGYCVIVAALGLAEGPLCLDSLGQWNYTFASTEGQYLLDTSTWSECTEPKHIVEWNVSLFSILLALGGIEFILCLIQVINGVLGGICGFCCSHQQQYDC
Host
Human
Theoretical MW (kDa)
21.64
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 6.3 ng/mL, messured by ELISA at 5 ug/mL.
Quality Control Testing
SEC-HPLC
SEC-HPLC
The purity of Human TM4SF1 VLP is greater than 95% as determined by SEC-HPLC.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human TM4SF1 VLP at 5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-TM4SF1 Antibody, hFc Tag with the EC50 of 6.3 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — TM4SF1
Entrez GeneID
4071Protein Accession#
P30408Gene Name
TM4SF1
Gene Alias
H-L6, L6, M3S1, TAAL6
Gene Description
transmembrane 4 L six family member 1
Omim ID
191155Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface antigen and is highly expressed in different carcinomas. [provided by RefSeq
Other Designations
membrane component, chromosome 3, surface marker 1|transmembrane 4 superfamily member 1|tumor-associated antigen L6
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