MICB (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human MICB, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-MICB Antibody, hFc Tag with the EC50 of 54.9 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
SEC-HPLC
Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
Tris-Bis PAGE
Human MICB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human MICB (Q29980-1, 23 a.a. - 298 a.a.) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAENVLGAKTWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYDGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSG
Host
Human
Theoretical MW (kDa)
58.21
Form
Liquid
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 54.9 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
Tris-Bis PAGE
Human MICB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS pH 7.4
Storage Instruction
Store at -80°C for 12 Month.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human MICB, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-MICB Antibody, hFc Tag with the EC50 of 54.9 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). -
Gene Info — MICB
Entrez GeneID
4277Protein Accession#
Q29980-1Gene Name
MICB
Gene Alias
PERB11.2
Gene Description
MHC class I polypeptide-related sequence B
Omim ID
602436Gene Ontology
HyperlinkGene Summary
This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. [provided by RefSeq
Other Designations
MHC class I chain-related protein B|MHC class I mic-B antigen|MHC class I molecule|MHC class I-like molecule PERB11.2-IMX|OTTHUMP00000029089|OTTHUMP00000035091|OTTHUMP00000035588|stress inducible class I homolog
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Interactome
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Pathway
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Disease
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