DKK1 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human DKK1, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 5.1 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human DKK1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human DKK1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human DKK1 (O94907, 32 a.a. - 266 a.a.) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
TLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTKGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQRH
Host
Human
Theoretical MW (kDa)
26.87
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 5.1 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human DKK1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human DKK1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human DKK1, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 5.1 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — DKK1
Entrez GeneID
22943Protein Accession#
O94907Gene Name
DKK1
Gene Alias
DKK-1, SK
Gene Description
dickkopf homolog 1 (Xenopus laevis)
Omim ID
605189Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq
Other Designations
OTTHUMP00000019617|dickkopf homolog 1|dickkopf related protein-1|dickkopf-1 like
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Interactome
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Pathway
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Disease
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