VEGF121 (Human) Recombinant Protein
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Specification
Product Description
Human VEGF121 (P15692-9, 27 a.a. - 147 a.a.) partial recombinant protein expressed in HEK293 cells.
Sequence
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR
Host
Human
Theoretical MW (kDa)
14.07
Form
Liquid
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 21.7 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human VEGF121 Protein is greater than 90% as determined by SEC-HPLC.
Tris-Bis PAGE
Human VEGF121 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS pH 7.4
Storage Instruction
Store at -80°C for 12 Month.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human VEGF121 at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 21.7 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — VEGFA
Entrez GeneID
7422Protein Accession#
P15692-9Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
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Interactome
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