GCDH (Human) Recombinant Protein
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Specification
Product Description
Human GCDH (Q92947, 45 a.a. - 438 a.a.) partial recombinant protein with His tag at N-terminus expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFTASK
Host
Escherichia coli
Theoretical MW (kDa)
45.8
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90.0% by SDS-PAGE
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20mM Tris-HCl pH 8.0 (0.2 M NaCl, 5 mM DTT and 20% glycerol)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — GCDH
Entrez GeneID
2639Protein Accession#
Q92947Gene Name
GCDH
Gene Alias
ACAD5, GCD
Gene Description
glutaryl-Coenzyme A dehydrogenase
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
glutaryl-CoA dehydrogenase, mitochondrial
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Interactome
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Pathway
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Disease
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