Ccl2 (Rat) Recombinant Protein
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Specification
Product Description
Rat Ccl2 (P14844, 24 a.a. - 148 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
QPDAVNAPLTCCYSFTGKMIPMSRLENYKRITSSRCPKEAVVFVTKLKREICADPNKEWVQKYIRKLDQNQVRSETTVFYKIASTLRTSAPLNVNLTHKSEANASTLFSTTTSSTSVEVTSMTEN
Host
Escherichia coli
Theoretical MW (kDa)
14.1
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98.0% by SDS-PAGE
Activity
The ED50 is 1 - 10 ng/mL was determined by measuring the dose dependent chemotaxis with human THP-1 cells, corresponding to a specific activity of 100000 - 1000000 IU/mg. The optimal concentration should be determined for each specific application by an initial dose-response assay.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Ccl2
Entrez GeneID
24770Protein Accession#
P14844Gene Name
Ccl2
Gene Alias
MCP-1, Scya2, Sigje
Gene Description
chemokine (C-C motif) ligand 2
Gene Ontology
HyperlinkOther Designations
monocyte chemoattractant protein-1|monocyte chemotactic protein 1|small inducible cytokine A2|small inducible gene JE
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