TGFB2 (Human) Recombinant Protein
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Specification
Product Description
Human TGFB2 recombinant protein expressed in HEK293 cells.
Host
Human
Theoretical MW (kDa)
25
Form
Lyophilized
Preparation Method
Mammalian cell (HEK 293) expression system
Purification
chromatographic
Purity
> 97% as determined by HPLC and SDS-PAGE.
Activity
ED50 is 0.16 ng/mL, measured by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).
Storage Buffer
Lyophilized from a solution containing 50 mM sodium acetate, pH 4.5. Reconstitute the lyophilized powder in 20% ethanol, 50 mM sodium acetate, 75 mM acetic acid.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — TGFB2
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Interactome
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Pathway
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Disease
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