Spp1 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Spp1 partial recombinant protein with His tag in C-terminus expressed in HEK293 cells.
Sequence
DGSLPVKVTDSGSSEEKLYSLHPDPIATWLVPDPSQKQNLLAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDRYLKFRISHELESSSSEVNHHHHHH
Host
Human
Theoretical MW (kDa)
31.8
Specificity
SPP1 Mouse
Form
Liquid
Preparation Method
Mammalian cell (HEK 293) expression system
Purification
chromatographic
Purity
> 90% as determined by SDS-PAGE.
Activity
ED50 < 1.5 ug/mL, measured by the ability of the immobilized protein to support the adhesion of HEK293 HEK cells when the cells are added to mouse SPP1 coated plates.
Storage Buffer
Solution (0.25 mg/mL) containing 1X PBS, pH 7.4, 10% glycerol.
Storage Instruction
Store at 4°C for 2-4 weeks and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Spp1
Entrez GeneID
20750Protein Accession#
P10923Gene Name
Spp1
Gene Alias
AA960535, AI790405, Apl-1, BNSP, BSPI, Bsp, ETA-1, Eta, OP, Opn, Opnl, Ric, Spp-1, minopontin
Gene Description
secreted phosphoprotein 1
Gene Ontology
HyperlinkOther Designations
44 kDa bone phosphoprotein|44kDa bone phosphoprotein|OTTMUSP00000029095|OTTMUSP00000029097|OTTMUSP00000029098|bone sialoprotein 1|osteopontin|osteopontin-like protein
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