Shh (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Shh recombinant protein expressed in Escherichia coli.
Sequence
MIIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG
Host
Escherichia coli
Theoretical MW (kDa)
19.8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 97% as determined by SDS-PAGE.
Activity
ED50 is 0.48-0.72 ug/mL, measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts.
Isotype
Escherichia Coli.
Storage Buffer
Lyophilized from a solution containing 10 mM Na2PO4, pH 7.5. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C.
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Shh
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