Pdgfa (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Pdgfa (P20033) recombinant protein expressed in Escherichia coli.
Sequence
MSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATSNLNPDHREEETGRRRESGKNRKRKRLKPT.
Host
Escherichia coli
Theoretical MW (kDa)
28.9
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 97% by HPLC and SDS PAGE
Activity
Established by the dose-dependent stimulation of Balb/c 3T3 cells proliferation. The expected ED50 for this effect is 8-10 ng/mL corresponding to a specific activity of 100,000-125,000 IU/mg.
Storage Buffer
The protein was lyophilized with no additives.
Storage Instruction
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Pdgfa
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