IL32 (Human) Recombinant protein
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Specification
Product Description
Human IL32 (P24001) recombinant protein expressed in Escherichia Coli.
Sequence
MCFPKVLSDD MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA YYEEQHPELT PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKSYG APRGDKEELT PQKCSEPQSS K.
Host
Escherichia coli
Theoretical MW (kDa)
14.9
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 97% by SDS-PAGE.
> 97% by RP-HPLC.Activity
Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha in the human THP-1 monocytic cell line.
Storage Buffer
Lyophilized from 50mM sodium Phosphate buffer, pH 7.5
Storage Instruction
Lyophilized although stable at room temperature for 3 weeks. should be stored desiccated below -20°C. Upon reconstitution should be stored at 4°C between 2-7 days and for future use below -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — IL32
Entrez GeneID
9235Protein Accession#
P24001Gene Name
IL32
Gene Alias
IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd
Gene Description
interleukin 32
Omim ID
606001Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
natural killer cell transcript 4|natural killer cells protein 4|tumor necrosis factor alpha-inducing factor
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