GH1 (Human) Recombinant Protein
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Specification
Product Description
Human GH1 recombinant protein with His tag in N-terminus expressed in Nicotiana benthamiana plant.
Sequence
HHHHHHFPTIPLSRPFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGFAG
Theoretical MW (kDa)
22.9
Form
Lyophilized
Preparation Method
Nicotiana benthamiana plant expression system
Purity
> 97% as determined by SDS-PAGE.
Activity
ED50 < 0.04-0.1 ng/mL, measured by cell proliferation using Nb2-11 cells.
Storage Buffer
Protein (1 mg/mL) was lyophilized from a solution containing 0.05M PBS, pH7.5. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — GH1
Entrez GeneID
2688Protein Accession#
P01241Gene Name
GH1
Gene Alias
GH, GH-N, GHN, hGH-N
Gene Description
growth hormone 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq
Other Designations
pituitary growth hormone
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Interactome
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