Gdf5 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Gdf5 recombinant protein expressed in Escherichia coli.
Sequence
APLANRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR
Host
Escherichia coli
Theoretical MW (kDa)
27.2
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 96% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 1.0 ug/mL, measured by inducing alkaline phosphatase production of murine ATDC5 cells, corresponding to a specific activity of > 1.0 x 103 IU/mg.
Storage Buffer
Lyophilized from a solution containing 30% Acetonitrile, 0.1% TFA. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Gdf5
Entrez GeneID
14563Protein Accession#
P43027Gene Name
Gdf5
Gene Alias
Cdmp-1, bp, brp
Gene Description
growth differentiation factor 5
Gene Ontology
HyperlinkOther Designations
Growth/differentiation factor 5 precursor (GDF-5)|OTTMUSP00000017076|brachypodism|cartilage-derived morphogenetic protein-1
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