FST (Human) Recombinant Protein
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Specification
Product Description
Human FST recombinant protein expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
31.5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 95% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 is 100-400 ng/mL, measured by the ability to neutralize ACTV inhibitory effect of mouse MPC-11 cells, corresponding to a specific activity of 2500-10000 units/mg in the presence of 7.5 ng/mL ACTV A.
Storage Buffer
Protein (1 mg/mL) was lyophilized with no additives. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — FST
Entrez GeneID
10468Protein Accession#
P19883Gene Name
FST
Gene Alias
FS
Gene Description
follistatin
Gene Ontology
HyperlinkGene Summary
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. [provided by RefSeq
Other Designations
follistatin isoform FST317
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Interactome
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Pathway
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Disease
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