FGF9 (Human) Recombinant Protein
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Specification
Product Description
Human FGF9 recombinant protein expressed in?Escherichia coli.
Sequence
APLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS
Host
Escherichia coli
Theoretical MW (kDa)
23.5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatography
Purity
> 95% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 0.5 ng/mL, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake), corresponding to a Specific Activity of 2 x 106Units/mg.
Storage Buffer
Protein(1 mg/mL) was lyophilized from a solution containing 1X PBS. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — FGF9
Entrez GeneID
2254Protein Accession#
P31371Gene Name
FGF9
Gene Alias
GAF, HBFG-9, MGC119914, MGC119915
Gene Description
fibroblast growth factor 9 (glia-activating factor)
Omim ID
600921Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000018804|fibroblast growth factor 9|glia-activating factor
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Interactome
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