Fgf2 (Rat) Recombinant Protein
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Specification
Product Description
Rat Fgf2 recombinant protein expressed in?Escherichia coli.
Sequence
MPALPEDGGGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Host
Escherichia coli
Theoretical MW (kDa)
16.4
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatography
Purity
> 95% as determined by SDS-PAGE.
Activity
ED50 is 0.903 ng/mL, measured by the dose-dependant proliferation of NR6R-3T3 cells, corresponding to a Specific Activity of 1.1 x 107Units/mg.
Storage Buffer
Protein(1 mg/mL) was lyophilized from a solution containing 5mM Na2PO4, pH7.5, 50mM NaCl. Reconstitute the lyophilized powder in ddH2O to 101 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Fgf2
Entrez GeneID
54250Protein Accession#
A0A7U3JW58Gene Name
Fgf2
Gene Alias
Fgf-2, bFGF
Gene Description
fibroblast growth factor 2
Gene Ontology
HyperlinkOther Designations
basic fibroblast growth factor
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