FGF1 (Human) Recombinant Protein
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Specification
Product Description
Human FGF1 recombinant protein expressed in Baculovirus cells.
Host
Viruses
Theoretical MW (kDa)
15.8
Form
Liquid
Preparation Method
Baculovirus expression system
Purification
Chromatography
Purity
> 95% as determined by (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Activity
ED50 <10 ng/mL, measured by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake), corresponding to a specific activity <1 x 105 IU/mg.
Storage Buffer
In 20mM Tris-HCl, pH 7.9 (20% glycerol, 100 mM KCl, 0.2 mM DTT).
Storage Instruction
Stored at 4°C for 2-4 weeks, should be stored at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — FGF1
Entrez GeneID
2246Protein Accession#
Q53ZZ1Gene Name
FGF1
Gene Alias
AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1
Gene Description
fibroblast growth factor 1 (acidic)
Omim ID
131220Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000066028|OTTHUMP00000066030|OTTHUMP00000066031|OTTHUMP00000174675|endothelial cell growth factor, alpha|endothelial cell growth factor, beta|heparin-binding growth factor 1
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