Ngf (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Ngf (P01139) recombinant protein expressed in Escherichia coli.
Sequence
MSSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG.
Host
Escherichia coli
Theoretical MW (kDa)
13.4
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% by HPLC and SDS PAGE
Activity
The Mouse b-NGF activity was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1, the ED50 for this effect is 0.2 ng/mL, corresponding to a Specific Activity of 5,000,000 units/mg.
Storage Buffer
The Recombinant Mouse b-NGF was lyophilized without additives.
Storage Instruction
Store at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
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Applications
Functional Study
SDS-PAGE
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Gene Info — Ngf
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