NGF (Human) Recombinant Protein
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Specification
Product Description
Human NGF (P01138, 122 a.a. - 239 a.a.) partial-length recombinant protein expressed in CHO cells.
Host
Human
Theoretical MW (kDa)
26.5
Form
Lyophilized
Preparation Method
CHO cell expression system
Purity
> 95% by HPLC and SDS PAGE
Activity
The ED50, calculated by the ability to stimulate chick E9 DRG neurite outgrowth was found to be > 1.0 ng/mL, corresponding to a specific activity of < 1 x 106 units/mg.
Storage Buffer
The protein was lyophilized from a 0.2um filtered solution in 20mM PB and 250mM NaCl, pH 7.0.
Storage Instruction
Store at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
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Applications
Functional Study
SDS-PAGE
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Gene Info — NGF
Entrez GeneID
4803Protein Accession#
P01138Gene Name
NGF
Gene Alias
Beta-NGF, HSAN5, MGC161426, MGC161428, NGFB
Gene Description
nerve growth factor (beta polypeptide)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq
Other Designations
OTTHUMP00000013653|beta-nerve growth factor|nerve growth factor, beta polypeptide|nerve growth factor, beta subunit
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