IGFBP4 (Human) Recombinant Protein
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Specification
Product Description
Human IGFBP4 (P22692, 22 a.a. - 258 a.a.) partial recombinant protein expressed in Sf9 cells.
Sequence
DEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFRE
Host
insect
Theoretical MW (kDa)
30
Form
Lyophilized
Preparation Method
Sf9 cell expression system
Purity
> 97% by SDS-PAGE
Activity
The ED50 is < 0.1 ug/mL as determined by the ability to inhibit IGF-II induced proliferation of MCF-7 cells, corresponding to a specific activity of > 1.0 × 104 IU/mg in the presence of 14 ng/mL of rHuIGF-II.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — IGFBP4
Entrez GeneID
3487Protein Accession#
P22692Gene Name
IGFBP4
Gene Alias
BP-4, HT29-IGFBP, IBP4, IGFBP-4
Gene Description
insulin-like growth factor binding protein 4
Omim ID
146733Gene Ontology
HyperlinkGene Summary
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq
Other Designations
IGF-binding protein 4|insulin-like growth factor-binding protein 4
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