PGK2 (Human) Recombinant Protein
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Specification
Product Description
Human PGK2 (P07205, 1 a.a. - 417 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MSLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASIPSIKYCLDNGAKAVVLMSHLGRPDGVPMPDKYSLAPVAVELKSLLGKDVLFLKDCVGAEVEKACANPAPGSVILLENLRFHVEEEGKGQDPSGKKIKAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPHKASGFLMKKELDYFAKALENPVRPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGMAYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIVKATSKGCITVIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKILPGVEALSNM
Host
Escherichia coli
Theoretical MW (kDa)
46.9
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Activity
Specific activity is > 500 unit/mg, in which one unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37°C.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In 20mM Tris-HCl pH 8.0 (20% glycerol, 0.1 M NaCl, 1 mM DTT)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — PGK2
Entrez GeneID
5232Protein Accession#
P07205Gene Name
PGK2
Gene Alias
PGK-2, PGKB, PGKPS, dJ417L20.2
Gene Description
phosphoglycerate kinase 2
Omim ID
172270Gene Ontology
HyperlinkGene Summary
The PGK2 gene encodes a testis-specific form of phosphoglycerate kinase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. See also PGK1 (MIM 311800), which is ubiquitously expressed in all somatic tissues and maps to chromosome Xq13.[supplied by OMIM
Other Designations
OTTHUMP00000016591|phosphoglycerate kinase 1, pseudogene 2|phosphoglycerate kinase autosomal pseudogene
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Glycolysis / Gluconeogenesis
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