CSTA (Human) Recombinant Protein
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Specification
Product Description
Human CSTA (P01040, 1 a.a. - 98 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MIPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAGDNKYMHLKVFKSLPGQNEDLVLTGYQVDKNKDDELTGF
Host
Escherichia coli
Theoretical MW (kDa)
13.1
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Activity
The IC50 value is < 1.0 nM, was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In 20mM Tris-HCl pH 8.0 (10% glycerol, 1 mM DTT)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — CSTA
Entrez GeneID
1475Protein Accession#
P01040Gene Name
CSTA
Gene Alias
STF1, STFA
Gene Description
cystatin A (stefin A)
Omim ID
184600Gene Ontology
HyperlinkGene Summary
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq
Other Designations
cystatin A|cystatin AS|stefin A
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