NMT1 (Human) Recombinant Protein
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SDS-PAGE analysis of NMT1 (Human) Recombinant Protein
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Specification
Product Description
Human NMT1 (NP_066565.1, 1 a.a. - 496 a.a ) full-length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMADESETAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKKKQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQFWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDTEKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWHRSLNPRKLIEVKFSHLSRNMTMQRTMKLYRLPETPKTAGLRPMETKDIPVVHQLLTRYLKQFHLTPVMSQEEVEHWFYPQENIIDTFVVENANGEVTDFLSFYTLPSTIMNHPTHKSLKAAYSFYNVHTQTPLLDLMSDALVLAKMKGFDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWKCPSMGAEKVGLVLQ
Host
Escherichia coli
Theoretical MW (kDa)
59.2
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 85% by SDS-PAGE
Quality Control Testing
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
SDS-PAGE analysis of NMT1 (Human) Recombinant Protein
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — NMT1
Entrez GeneID
4836Protein Accession#
P30419Gene Name
NMT1
Gene Alias
NMT
Gene Description
N-myristoyltransferase 1
Omim ID
160993Gene Ontology
HyperlinkGene Summary
Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM
Other Designations
alternative, short form NMT-S|long form, NMT-L|myristoyl-CoA:protein N-myristoyltransferase
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Interactome
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Disease
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