CLDN18 (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human CLDN18 (P56856-2, 1 a.a. - 261 a.a.) full-length recombinant protein expressed in 293 cells.
Sequence
MSTTTCQVVAFLLSILGLAGCIAATGMDMWSTQDLYDNPVTSVFQYEGLWRSCVRQSSGFTECRPYFTILGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV
Host
Mammals
Theoretical MW (kDa)
27.8
Form
Liquid
Preparation Method
Mammalian cell (293) expression system
Purity
> 75% by SDS-PAGE
Endotoxin Level
< 0.2 EU per 1 ug of protein (determined by gel clotting method)
Activity
Immobilized anti Claudin 18.2 antibody at 1 ug/mL (100 ul/Well). Dose response curve for CLDN18.2, hFc, Human with the EC50 of 17.60 ng/ml determined by ELISA.
Quality Control Testing
2 ug protein in Reducing condition, no heat treatment before loading and Non-Reducing condition SDS PAGE Stained with Coomassie Blue
Storage Buffer
In 20 mM PB, 500 mM NaCl, pH 7.0 (10% glycerol, 0.025% DDM, 0.005% CHS)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CLDN18
Entrez GeneID
51208Protein Accession#
P56856-2Gene Name
CLDN18
Gene Alias
SFTA5, SFTPJ
Gene Description
claudin 18
Omim ID
609210Gene Ontology
HyperlinkGene Summary
CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells (Niimi et al., 2001 [PubMed 11585919]).[supplied by OMIM
Other Designations
surfactant associated 5|surfactant associated protein J|surfactant, pulmonary associated protein J
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