MUC16 (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human MUC16 (Q8WXI7, 12660 a.a. - 12923 a.a.) partial recombinant protein with His-Avi tag at C-terminus expressed in Expi293 cells.
Sequence
GFTHWIPVPTSSTPGTSTVDLGSGTPSSLPSPTTAGPLLVPFTLNFTITNLKYEEDMHCPGSRKFNTTERVLQSLLGPMFKNTSVGPLYSGCRLTLLRSEKDGAATGVDAICTHRLDPKSPGVDREQLYWELSQLTNGIKELGPYTLDRNSLYVNGFTHQTSAPNTSTPGTSTVDLGTSGT_x005F_x005F_x005F_x000D__x005F_x000D_ 12841PSSLPSPTSAGPLLVPFTLNFTITNLQYEEDMHHPGSRKFNTTERVLQGLLGPMFKNTSV_x005F_x005F_x005F_x000D__x005F_x000D_ 12901GLLYSGCRLTLLRPEKNGAATGM
Host
Mammals
Theoretical MW (kDa)
50-80
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293) expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
Immobilized Human CA125, His & Avi Tag at 5.0 ug/mL (100 uL/Well). Dose response curve for Human MSLN with the EC50 of 3.78 ug/mL determined by ELISA.
Quality Control Testing
SDS-PAGE under reducing condition
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/mL
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MUC16
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Interactome
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Disease
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