Ccl3 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Ccl3 (Q5QNW0, 24 a.a. - 92 a.a.) partial recombinant protein expressed in HEK293 cells.
Sequence
APYGADTPTACCFSYSRKIPRQFIVDYFETSSLCSQPGVIFLTKRNRQICADSKETWVQEYITDLEL NA
Host
Human
Theoretical MW (kDa)
~ 7.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK 293) expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
EC50 of mouse MIP-1alpha/CCL3 < 100.0 ng/mL, measured by Ca2+ mobilization assay in CHO-K1/Galpha15/mCCR1 cells (human Galpha15 and mouse CCR1 stably expressed in CHO-K1 cells).
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Ccl3
Entrez GeneID
20302Protein Accession#
Q5QNW0Gene Name
Ccl3
Gene Alias
AI323804, G0S19-1, LD78alpha, MIP-1alpha, MIP1-(a), MIP1-alpha, Mip1a, Scya3
Gene Description
chemokine (C-C motif) ligand 3
Gene Ontology
HyperlinkOther Designations
MIP-1 alpha|MIP1 (a)|OTTMUSP00000000942|macrophage inflammatory protein-1alpha|small inducible cytokine A3
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