Il21 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Il21 (Q9ES17-1, 25 a.a. - 146 a.a.) partial recombinant protein expressed with an N-terminal Met in Escherichia coli.
Sequence
PDRLLIRLRHLIDIVEQLKIYENDLDPELLSAPQDVKGHCEHAAFACFQKAKLKPSNPGNNKTFIIDLVAQLRRRLPARRGGKKQKHIAKCPSCDSYEKRTPKEFLERLKWLLQKMIHQHLS
Host
Escherichia coli
Theoretical MW (kDa)
~ 15.1
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% as analyzed by SDS-PAGE.
Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
ED50 < 1.0 ng/mL, measured by the ability to stimulate the proliferation of human ANBL-6 cells,corresponding to a specific activity of > 1.0 × 106 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Il21
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