Il4 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Il4 (P07750, 21 a.a. - 140 a.a.) partial recombinant protein expressed in CHO cells.
Sequence
HIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS
Host
Mammals
Theoretical MW (kDa)
15
Form
Lyophilized
Preparation Method
Mammalian cell (CHO) expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/μg of protein by gel clotting method
Activity
ED50 < 2.0 ng/mL, measured in a cell proliferation assay using murine HT-2 cells, corresponding to a specific activity of > 5.0 × 105 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Il4
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