CXCL10 (Human) Recombinant Protein
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Specification
Product Description
Human CXCL10 (P02778, 22 a.a. - 98 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
MVPLSRTVRCTCISISNQPVNPRSLEKLEIIPASQFCPRVEIIATMKKKGEKRCLNPESKAIKNLLKAVSKERSKRSP
Host
Escherichia coli
Theoretical MW (kDa)
8.6
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/μg of protein by gel clotting method
Activity
ED50 < 0.2 ug/mL, measured by a cell proliferation assay of HUVEC cells in the presence of 2.5 ng/mL h-VEGF, corresponding to a specific activity of > 5.0 × 103 IU/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — CXCL10
Entrez GeneID
3627Protein Accession#
P02778Gene Name
CXCL10
Gene Alias
C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1
Gene Description
chemokine (C-X-C motif) ligand 10
Omim ID
147310Gene Ontology
HyperlinkGene Summary
This gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq
Other Designations
gamma IP10|interferon-inducible cytokine IP-10|protein 10 from interferon (gamma)-induced cell line|small inducible cytokine B10|small inducible cytokine subfamily B (Cys-X-Cys), member 10
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