SERPINF1 (Human) Recombinant Protein
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Specification
Product Description
Human SERPINF1 (P36955, 20 a.a. - 418 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
QNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVRSSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIHGTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVLTGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVTKFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLTGSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKLSYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRA_x005F_x005F_x005F_x000D__x005F_x000D__x000D_ 361GFEWNEDGAGTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP
Host
Escherichia coli
Theoretical MW (kDa)
44.4
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 97% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The ED50 was determined by the ability to enhance the adhesion of human Saos2 cells to bovine Collagen I coated plate is < 2.0 ng/ml, corresponding to a specific activity of > 5.0 x 105 IU/mg.
Storage Buffer
Lyophilized from sterile distilled Water up to 0.1 - 1.0 mg/ml
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — SERPINF1
Entrez GeneID
5176Protein Accession#
P36955Gene Name
SERPINF1
Gene Alias
EPC-1, PEDF, PIG35
Gene Description
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Omim ID
172860Gene Ontology
HyperlinkGene Summary
The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.[supplied by OMIM
Other Designations
pigment epithelium-derived factor|proliferation-inducing protein 35|serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
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Interactome
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Disease
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