Il2 (Rat) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
Il2 (Rat) Recombinant Protein (Cat # P6974) stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range is less or equal to 0.3 ng/mL.
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Specification
Product Description
Rat Il2 (NP_446288, 21 a.a. - 155 a.a.) partial recombinant protein expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
15.6
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method).
Activity
Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is less or equal to 0.3 ng/mL.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
Bioactivity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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Gene Info — Il2
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