Tnf (Rat) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
Tnf (Rat) Recombinant Protein stimulates cytotoxicity of the L929 mouse fibroblast cells. The ED50 range ≤ 0.2 ng/mL.
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Specification
Product Description
Rat Tnf (NP_038721, 80 a.a. - 235 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
Host
Rat
Theoretical MW (kDa)
19.9
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
> 1 EU per 1ug of protein (determined by LAL method)
Activity
Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2 ng/ml.
Quality Control Testing
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (1 mM DTT, 10% glycerol)
Storage Instruction
Stored at 2°C to 8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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Gene Info — Tnf
Entrez GeneID
24835Protein Accession#
NP_036807Gene Name
Tnf
Gene Alias
MGC124630, RATTNF, TNF-alpha, Tnfa
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
tumor necrosis factor superfamily member 2|tumor necrosis factor superfamily, member 2|tumor necrosis factor alpha|tumor necrosis factor superfamily, member 2|tumor necrosis factor, alpha (cachetin)|tumor necrosis factor-alpha
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