HAO1 (Human) Recombinant Protein
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More Files
- More Functions
15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.
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Specification
Product Description
Human Hydroxyacid Oxidase-1/HAO-1 protein (NP_060015, 1 a.a.-370 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
45
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU/ug
Activity
Specific activity is > 3,000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25°C.
Quality Control Testing
15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.
15% SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, 0.5M NaCl, pH 8.0 (20% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — HAO1
Entrez GeneID
54363Protein Accession#
Q9UJM8Gene Name
HAO1
Gene Alias
GOX, GOX1, HAOX1, MGC142225, MGC142227
Gene Description
hydroxyacid oxidase (glycolate oxidase) 1
Omim ID
605023Gene Ontology
HyperlinkGene Summary
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq
Other Designations
(S)-2-hydroxy-acid oxidase|OTTHUMP00000030231|glycolate oxidase|hydroxyacid oxidase 1
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Interactome
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Pathway
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