NCR3 (Human) Recombinant Protein
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Specification
Product Description
Human NCR3 (NP_001138938.1, 19 a.a. - 138 a.a.) partial recombinant protein with Fc, 6x His tag at C-terminal expressed in HEK293 cells.
Host
Human
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Ni-sepharose purification
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug of the protein by LAL method.
Activity
Measured by the binding ability in a functional ELISA. Immobilized recombinant Human B7-H6 at 2 ug/mL (100 uL/well) can bind NCR3 (Human) Recombinant Protein (Cat # P6791), the ED50 of Human NCR3 is 66.43 g/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
NCR3 (Human) Recombinant Protein (Cat # P6791) was determined by SDS-PAGE with Coomassie Blue, showing a band at 54-60 kDa.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 7.4.
Storage Instruction
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution to a concentration of 0.1-0.5 mg/mL in sterile distilled water, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
SDS-PAGE
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Gene Info — NCR3
Entrez GeneID
259197Protein Accession#
NP_001138938.1Gene Name
NCR3
Gene Alias
1C7, CD337, LY117, MALS, NKp30
Gene Description
natural cytotoxicity triggering receptor 3
Omim ID
611550Gene Ontology
HyperlinkGene Summary
Natural cytotoxicity receptors (NCRs), such as NCR3, are activating natural killer (NK) cell receptors that belong to the immunoglobulin (Ig) superfamily. NCR3 is expressed in all resting and activated NK cells and forms a complex with CD3-zeta (CD3Z, or CD247; MIM 186780) (Sato et al., 2001 [PubMed 11782277]).[supplied by OMIM
Other Designations
OTTHUMP00000029162|OTTHUMP00000038859|OTTHUMP00000038863|lymphocyte antigen 117
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