PIP4K2B (Human) Recombinant Protein
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Specification
Product Description
Human PIP4K2B (NP_003550, 1 a.a. - 416 a.a.) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.95
Activity
The activity was determined by ADP-Glo Assay. The enzyme was incubated with Lipid substrate and Mn/ATP. The phosphorylation was detected by the ADP-Glo Kinase Assay technology (luminescent ADP detection assay). Substrate: PI (5) P and Phosphatidylserine, ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — PIP4K2B
Entrez GeneID
8396Protein Accession#
NP_003550Gene Name
PIP4K2B
Gene Alias
PI5P4KB, PIP5K2B, PIP5KIIB, PIP5KIIbeta
Gene Description
phosphatidylinositol-5-phosphate 4-kinase, type II, beta
Omim ID
603261Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor. [provided by RefSeq
Other Designations
1-phosphatidylinositol-4-phosphate kinase|PTDINS(4)P-5-kinase|diphosphoinositide kinase|phosphatidylinositol-4-phosphate 5-kinase, type II, beta
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