RPS6KA3 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human RPS6KA3 (NP_004577.1, 1 a.a. - 740 a.a.) full length recombinant protein with GST-tag at N-terminal using baculovirus expression system.
Host
Viruses
Form
Liquid
Preparation Method
Baculovirus expression system.
Purification
Glutathione sepharose chromatography.
Purity
0.6
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. Substrate: S6K peptide (N-FL), ATP: 100 uM.
Quality Control Testing
The purity was assessed by SDS-PAGE/CBB staining.
Storage Buffer
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Storage Instruction
Stored at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
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Gene Info — RPS6KA3
Entrez GeneID
6197Protein Accession#
NP_004577.1Gene Name
RPS6KA3
Gene Alias
CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, p90-RSK2, pp90RSK2
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq
Other Designations
OTTHUMP00000023036|insulin-stimulated protein kinase 1|mental retardation, X-linked 19|ribosomal protein S6 kinase, 90kD, polypeptide 3
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