SGK2 (S356D) (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human SGK2 (NP_733794.1, 1 a.a. - 367 a.a.) S356D mutant full length recombinant protein with GST tag co-expressed with His-tagged PDPK1 (NP_002604.1, 1 a.a. - 556 a.a.) full length recombinant protein in Baculovirus infected Sf21 cells.
Theoretical MW (kDa)
68
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
97 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : SGKtide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — SGK2
Entrez GeneID
10110Protein Accession#
NP_733794.1Gene Name
SGK2
Gene Alias
H-SGK2, dJ138B7.2
Gene Description
serum/glucocorticoid regulated kinase 2
Omim ID
607589Gene Ontology
HyperlinkGene Summary
This gene encodes a serine/threonine protein kinase. Although this gene product is similar to serum- and glucocorticoid-induced protein kinase (SGK), this gene is not induced by serum or glucocorticoids. This gene is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK. Two alternate transcripts encoding two different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000031702|OTTHUMP00000031703|OTTHUMP00000031705
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