CDK5/CDK5R1 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human CDK5 (NP_004926.1, 1 a.a. - 292 a.a.) full-length recombinant protein with GST tag co-expressed with CDK5R1 (NP_003876.1, 99 a.a. - 307 a.a.) expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
60
Form
Liquid
Preparation Method
Baculovirus expression system
Purification
Glutathione sepharose chromatography and anion exchange chromatography
Purity
99 % by SDS-PAGE/CBB staining.
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : Modified Histone H1. ATP: 100 µM.Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CDK5
Entrez GeneID
1020Protein Accession#
NP_004926.1 (Gene ID : 1020); NP_003876.1 (Gene ID : 8851)Gene Name
CDK5
Gene Alias
PSSALRE
Gene Description
cyclin-dependent kinase 5
Omim ID
123831Gene Ontology
HyperlinkOther Designations
protein kinase CDK5 splicing
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Gene Info — CDK5R1
Entrez GeneID
8851Protein Accession#
NP_004926.1 (Gene ID : 1020); NP_003876.1 (Gene ID : 8851)Gene Name
CDK5R1
Gene Alias
CDK5P35, CDK5R, MGC33831, NCK5A, p23, p25, p35, p35nck5a
Gene Description
cyclin-dependent kinase 5, regulatory subunit 1 (p35)
Omim ID
603460Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq
Other Designations
CDK5 activator 1|TPKII regulatory subunit|cyclin-dependent kinase 5 activator 1|cyclin-dependent kinase 5, regulatory subunit 1|neuronal CDK5 activator|regulatory partner for CDK5 kinase|tau protein kinase II 23kDa subunit
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