CSNK1G1 (Human) Recombinant Protein
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Specification
Product Description
Human CSNK1G1 (NP_071331.2, 1 a.a. - 422 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
76
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
95 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CSNK1G1
Entrez GeneID
53944Protein Accession#
NP_071331.2Gene Name
CSNK1G1
Gene Alias
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Gene Description
casein kinase 1, gamma 1
Omim ID
606274Gene Ontology
HyperlinkGene Summary
Casein kinase I is the most abundant serine/threonine kinase in eukaryotic cell extracts. Multiple isoforms of the enzyme exist. The gamma-1 isoform is involved in growth and morphogenesis of eukaryotic cells.[supplied by OMIM
Other Designations
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