PAK1 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen):
1. Different concentrations of PAK1 were incubated in a buffer containing 50 mM HEPES pH 7.5, 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (SER/THR 14, Invitrogen) at RT for 1 hour.
2. Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at RT.
3. Fluorescence was then detected using λexc=460±40 nm and λem=528±20 nm filters.
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Specification
Product Description
Human PAK1 kinase domain (Q13153, 248 a.a. - 545 a.a.) partial recombinant protein expressed in Escherichia coli. The recombinant protein does not have the inhibitory switch domain and has high specific activity.
Sequence
GPHMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH
The first 4 residues GPHM are from Turbo3C Protease cleavage site. The underlined T is phosphorylated T423.Host
Escherichia coli
Theoretical MW (kDa)
33.7
Form
Liquid
Preparation Method
Escherichia coli expression system
Concentration
1 mg/ml
Activity
Specific activity: 32,638 pmoles/min/ug
Quality Control Testing
Loading 8 ug protein in SDS-PAGE
Storage Buffer
In 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PAK1
Entrez GeneID
5058Protein Accession#
Q13153Gene Name
PAK1
Gene Alias
MGC130000, MGC130001, PAKalpha
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 1
Omim ID
602590Gene Ontology
HyperlinkGene Summary
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
STE20 homolog, yeast|p21-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
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Interactome
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Pathway
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Disease
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Publication Reference
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Pak1 Kinase Maintains Apical Membrane Identity in Epithelia.
Aguilar-Aragon M, Elbediwy A, Foglizzo V, Fletcher GC, Li VSW, Thompson BJ.
Cell Reports 2018 Feb; 22:1639.
Application：In vitro kinase assay, PAK1 protein.
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Rho-Kinase Planar Polarization at Tissue Boundaries Depends on Phospho-regulation of Membrane Residence Time.
Sidor C, Stevens TJ, Jin L, Boulanger J, Röper K.
The Annals of Thoracic Surgery 1991 May; 51(5):848.
Application：KA, Recombinant protein.
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Pak1 Kinase Maintains Apical Membrane Identity in Epithelia.
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