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Result of activity analysis
Result of activity analysis
50 ug of salmon sperm DNA was incubated with the indicated units of TurboNuclease at 37°C for 30 minutes in a buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. DNA digestion was check by agarose gel electrophoresis.
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Specification
Product Description
Serratia macescens extracellular endonuclease expressed in Escherichia coli.
Biological function
TurboNuclease (aka Benzonase) is a homodimer endonuclease nonspecifically hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5'-phosphorylated oligonucleotides of 1-4 bases in length.
Host
Escherichia coli
Theoretical MW (kDa)
27 (monomer)
Form
Liquid
Preparation Method
Escherichia coli expression system
Concentration
250 Units/ul
Purity
> 99% (no detectable protease activity)
Endotoxin Level
< 0.1 EU/1,000 units as determined by Endo-Safe LAL Assay.
Activity
1 unit of TurboNuclease converts 1.0 OD260 of salmon sperm DNA into acid-soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 ug of salmon sperm DNA into oligonucleotides. TurboNuclease has similar specific activity as Benzonase.
Quality Control Testing
Loading 3 ug protein in SDS-PAGE
Recommend Usage
Used to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
Storage Buffer
In 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl2 and 50% Glycerol
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Enzyme Activity
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Publication Reference
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Tail proteins of phage SU10 reorganize into the nozzle for genome delivery.
Marta Šiborová, Tibor Füzik, Michaela Procházková, Jiří Nováček, Martin Benešík, Anders S Nilsson, Pavel Plevka.
Nature Communications 2022 Sep; 13(1):5622.
Application：Enzyme , Bacteria, E.coli.
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H3K9 dimethylation safeguards cancer cells against activation of the interferon pathway.
Anne Meldgaard Hansen, Ying Ge, Mikkel Bruhn Schuster, Sachin Pundhir, Janus Schou Jakobsen, Adrija Kalvisa, Marta Cecylia Tapia, Sandra Gordon, Francesca Ambri, Frederik Otzen Bagger, Deo Pandey, Kristian Helin, Bo Torben Porse.
Science Advances 2022 Mar; 8(11):eabf8627.
Application：Enzyme, Mouse, Mouse acute myeloid leukemia.
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Tail proteins of phage SU10 reorganize into the nozzle for genome delivery.
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