-
More Files
- More Functions
Result of activity analysis
Result of activity analysis
A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with Turbo3C Protease (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 14 mM b-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa.
-
Specification
Product Description
Human rhinovirus (HRV) 3C protease with GST and His tags expressed in Escherichia coli.
Biological function
Turbo3C Protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly.
Host
Escherichia coli
Theoretical MW (kDa)
47
Specificity
No non-specific cleavage of target cleavage protein is observed at protease-to-control cleavage protein ratio of 1:10.
Form
Liquid
Preparation Method
Escherichia coli expression system
Concentration
2 mg/ml
Purity
> 99% by SDS-PAGE
Endotoxin Level
< 5 EU/mg by Charles River EndoSafe-PTS
Activity
> 1 Units/ug. 1 Units of Turbo3C (HRV3C) Protease cleaves > 95% of 100 ug of target cleavage protein at 4°C for 16 hours.
Recommend Usage
Application that require protein digestion.
Storage Buffer
In 25 mM Tris-HCl, pH8.0, 50 mM NaCl, 1 mM TCEP, 50% glycerol
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
-
Applications
Enzyme Activity
-
Publication Reference
-
Characterisation of the enzyme transport path between shipworms and their bacterial symbionts.
Giovanna Pesante, Federico Sabbadin, Luisa Elias, Clare Steele-King, J Reuben Shipway, Adam A Dowle, Yi Li, Marta Busse-Wicher, Paul Dupree, Katrin Besser, Simon M Cragg, Neil C Bruce, Simon J McQueen-Mason.
BMC Biology 2021 Nov; 19(1):233.
Application：Func, Strep-tag.
-
Soluble expression of IGF1 fused to DsbA in SHuffle™ T7 strain: optimization of expression and purification by Box-Behnken design.
Emamipour N, Vossoughi M, Mahboudi F, Golkar M, Fard-Esfahani P.
Applied Microbiology and Biotechnology 2019 Apr; 103(8):3393.
Application：Func, DsbA-IGF1 protein.
-
Characterisation of the enzyme transport path between shipworms and their bacterial symbionts.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com