Reverse Transcriptase (HIV) Recombinant Protein
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Specification
Product Description
Chromatographically purified dimeric form with M.W. of 66 kDa and 51 kDa.
Biological function
Reverse transcriptases are enzymes encoded in retroviruses viral genome. The enzyme is responsible for transcription of the viral RNA to produce a dsDNA that can be inserted into the host genome.
Host
Escherichia coli
Form
Liquid
Preparation Method
Escherichia coli expression system
Activity
>=5,000 units per mg protein. One Unit incorporates 1 nmole of tritiated d-TMP into acid precipitable products using poly(A)/oligo(dT)12-18 as the template/primer in 20 minutes at 37°C, pH 8.3.
Recommend Usage
Reverse Transcriptase Assay. HIV reverse transcriptase is used for research on the AIDS primer. However it can be substituted for AMV reverse transcriptase, which is mainly used to transcribe mRNA into double stranded cDNA, that can be inserted into prokaryotic vectors. The enzyme can also be used with either single stranded DNA or RNA templates to make probes for use in hybridization experiments. It can be used for labeling the termini of DNA fragments with protruding 5' termini. The enzyme can also be used to sequence DNAs by the dideoxy chain termination method of Sanger when the Klenow fragment of E. coli DNA polymerase I, or the T7 DNA polymerase yield unsatisfactory results.
Storage Buffer
In 10 mM potassium phosphate, pH 7.4 (1 mM DTT and 20% glycerol).
Storage Instruction
Store at -20°C.
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Applications
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