Lep (Rat) Recombinant Protein
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Lane 1: non-reducing conditions
Lane 2: reducing conditions
Result of activity analysis
Result of activity analysis
Serial dilutions of rat Lep were added to Baf/3 cells. After 44 hours cell proliferation was measured and the linear portion of the curve was used to determine the ED50.
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Specification
Product Description
Rat Lep recombinant protein expressed in Escherichia coli.
Sequence
MVPIHKVQDDTKTLIKTIVTRINDISHTQSVSARQRVTGLDFIPGLHPILSLSKMDQTLAVYQQILTSLPSQNVLQIAHDLENLRDLLHLLAFSKSCSLPQTRGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDLSPEC
Host
Escherichia coli
Theoretical MW (kDa)
16.2
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the leptin-dependent stimulation of mouse BaF3 indicator cells. The expected ED50 for this effect is 4-6.7 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
Lyophilized from 0.1% TFA
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Lep
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