Igf1 (Rat) Recombinant Protein
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Lane 1: non-reducing conditions
Lane 2: reducing conditions
Result of activity analysis
Result of activity analysis
FDC-P1 cells were cultured with 0 to 250 ng/mL rat Igf1. Cell proliferation was measured after 48 hours and the linear portion of the curve was us used to calculate the ED50.
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Specification
Product Description
Rat Igf1 recombinant protein expressed in Escherichia coli.
Sequence
GPETLCGAELVDALQFVCGPRGFYFNKPTGYGSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKSA
Host
Escherichia coli
Theoretical MW (kDa)
7.7
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose dependent proliferation of FDC-P1 cells. The expected ED50 for this effect is 61-91 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
No additive
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Igf1
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