Pdgfb/Pdgfb (Mouse) Recombinant Protein
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Result of activity analysis
Result of activity analysis
Serial dilutions of mouse PDGF-BB, starting at 100 ng/mL, were added to NIH 3T3 cells. After 40 hours, cell viability was measured and the linear portion of the curve was us used to calculate the ED50.
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Specification
Product Description
Mouse Pdgfb (homodimer) recombinant protein expressed in Escherichia coli.
Sequence
MSLGSLAAAEPAVIAECKTRTEVFQISRNLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRASQVQMRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETIVTPRPVT
Host
Escherichia coli
Theoretical MW (kDa)
24.6
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent proliferation in mouse 3T3 fibroblasts. The expected ED50 for this effect is 0.2-0.3 ng/mL.
Storage Buffer
Lyophilized from 10 mM sodium citrate, pH 3.0
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Pdgfb
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