CXCL12 (Alpha) (Human) Recombinant Protein
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Specification
Product Description
Human CXCL12 (alpha) (P48061-2) recombinant protein expressed in Escherichia coli.
Sequence
KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK
Host
Escherichia coli
Theoretical MW (kDa)
8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the ability to chemoattract human peripheral T cells and is detectable starting at 5 ng/mL
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
No additive
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CXCL12
Entrez GeneID
6387Protein Accession#
P48061-2Gene Name
CXCL12
Gene Alias
PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1
Gene Description
chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
Gene Ontology
HyperlinkGene Summary
For background information on chemokines, see CXCL1 (MIM 155730). Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF (see MIM 191160), or IL1 (see MIM 147760). The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group.[supplied by OMIM
Other Designations
OTTHUMP00000019491|stromal cell-derived factor 1 delta|stromal cell-derived factor 1 gamma|stromal cell-derived factor 1a
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