ALKBH2 (Human) Recombinant Protein
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Specification
Product Description
Human ALKBH2 (NP_001138847, 1 a.a. - 261 a.a.) full-length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMDRFLVKGAQGGLLRKQEEQEPTGEEPAVLGGDKESTRKRPRREAPGNGGHSAGPSWRHIRAEGLDCSYTVLFGKAEADEIFQELEKEVEYFTGALARVQVFGKWHSVPRKQATYGDAGLTYTFSGLTLSPKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKILLTKK
Host
Escherichia coli
Theoretical MW (kDa)
31.4
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
0.5 mg/mL
Purity
> 90% by SDS-PAGE
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (1 mM DTT, 30% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — ALKBH2
Entrez GeneID
121642Protein Accession#
NP_001138847Gene Name
ALKBH2
Gene Alias
ABH2, MGC90512, hABH2
Gene Description
alkB, alkylation repair homolog 2 (E. coli)
Omim ID
610602Gene Ontology
HyperlinkGene Summary
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM
Other Designations
2OG-Fe(II) oxy DC1|alkB, alkylation repair homolog 2
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Interactome
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Disease
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