NCF1 (Human) Recombinant Protein
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Specification
Product Description
Human NCF1 (AAH02816, 1 a.a. - 390 a.a.) full-length recombinant protein with His tag at C-terminal expressed in Escherichia coli.
Sequence
MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAVVEHHHHHH
Host
Escherichia coli
Theoretical MW (kDa)
45.7
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
0.5 mg/mL
Purity
> 90% by SDS-PAGE
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM Tris-HCl buffer, 0.1 M NaCl, pH 8.0 (1 mM DTT, 20% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — NCF1
Entrez GeneID
653361Protein Accession#
AAH02816Gene Name
NCF1
Gene Alias
FLJ79451, NCF1A, NOXO2, SH3PXD1A, p47phox
Gene Description
neutrophil cytosolic factor 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq
Other Designations
NADPH oxidase organizer 2
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